After discovering a mysterious orb in another part of the galaxy, Peter Quill from Earth, is now the main target of a manhunt led by the genocidal villain Ronan the Accuser. Being hunted across the galaxy, Quill gets lumped together with a group of misfits that need to learn how to get along before they can become the "Guardians of the Galaxy".

After saving Xandar from Ronan's wrath, the Guardians are now recognized as heroes. Now the team must help their leader Star Lord (Chris Pratt) uncover the truth behind his true heritage. Along the way, old foes turn to allies and betrayal is blooming. And the Guardians find that they are up against a devastating new menace who is out to rule the galaxy.